Molecular and epidemiologyical analysis of a Campylobacter jejuni outbreak in China, 2018

Molecular and epidemiologyical analysis of a Campylobacter jejuni outbreak in China, 2018

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Introduction: Campylobacter spp.is the most common gastrointestinal pathogen worldwide with a very low reported incidence in China.In April 2018, one 36 cases of diarrhea outbreak occurred in a high school in Beijing after a trip to another province in Southern China.The investigation for the enteric pathogen infection was conducted to identify the cause.

Methodology: Eighteen stool specimens from 11 diarrheal patients and 3 close contacts were collected and tested for 16 enteric bacterial and viral pathogens using real-time PCR methods.Multilocus Sequence Typing (MLST), pulsed-field gel electrophoresis (PFGE) and antimicrobial susceptibility testing were applied to C.jejuni isolates in this outbreak in order Originals High Rise Wide Leg Crop to characterize the incident.Results: Ten (90.

9%) of 11 stool specimens from diarrheal patients were positive for Campylobacter jejuni, confirmed as the cause of outbreak.Eight C.jejuni strains were obtained and produced 2 sequence types (STs) and 3 PFGE patterns.Six of them had the same ST (ST2274) Ball - Miscellaneous and PFGE pattern (SMA001).

The dominant outbreak strain with an identical subtyping profile was clustered with most chicken isolates from national MLST and PulseNet Campylobacter database.The entire 8 isolates were multi-drug resistant, with the dominant resistance pattern of nalidixic acid, tetracycline and ciprofloxacin combined, except for 2 isolates resistant to florfenicol.Conclusion: Molecular typing confirmed that most of the cases belonged to a clonal cluster supporting the hypothesis of a common source; however, the source was not identified.This was the first recognized Campylobacteriosis outbreak in China among 20 years.